How to Calculate Molarity
Molarity formula explained for the bench: mass = M × V × MW, worked examples for NaCl, Tris and EDTA, hydrate traps and a free molarity calculator.
Worked examples for the calculations every lab runs. Each guide links to a free BenchMate calculator so you can check your numbers.
Molarity formula explained for the bench: mass = M × V × MW, worked examples for NaCl, Tris and EDTA, hydrate traps and a free molarity calculator.
How to use C1V1 = C2V2 for lab dilutions: step-by-step examples, unit traps, 10X to 1X buffers, and when the equation does not apply.
How to plan a serial dilution: dilution factor, 1:10 and 1:2 series tables, CFU back-calculation and pipetting errors that compound.
Step-by-step buffer preparation: choosing a buffer by pKa, 1X PBS and 1 M Tris-HCl recipes, temperature effects on Tris pH, and pH meter habits.
What % w/v, % v/v and % w/w mean, how to make 70% ethanol, 10% SDS and 1% agarose, and how to convert percent to molarity.
Convert DNA and RNA concentration from ng/µL to nM, pmol and copy number. A260 basics, purity ratios, primer and plasmid examples.