Free PDF · printable
The Bench Starter Kit
Six one-page cheat sheets for the calculations you do every week. Print them, pin them above the bench, stop re-deriving C1V1 at 7 pm.
Built from the NexagenLabs guides and checked against the same numbers BenchMate uses.
Molarity and mass
mass = M × V × MW, hydrate MWs for common salts, purity correction.
C1V1 dilutions
Rearranged forms, unit-match table, when to do an intermediate dilution.
Serial dilutions and CFU
Dilution factor, 10-fold and 2-fold plate layouts, CFU/mL back-calculation.
Buffers
pKa table for common buffers, 1X PBS recipe with hydrate swaps, Tris temperature shift.
Percent solutions
w/v, v/v, w/w, percent to molarity, 70% ethanol, concentrated acid molarity.
Nucleic acids
A260 factors, purity ratios, ng/µL to nM and copies, ligation insert maths.